+20 Questions on Glycolysis
20 multiple choice questions on Glycolysis, numbered with answers at the end.
Multiple Choice Questions: Glycolysis
1. What is glycolysis?
A) The breakdown of glucose into pyruvate
B) The synthesis of glucose from pyruvate
C) The production of oxygen from water
D) The storage of glucose in cells
2. Where in the cell does glycolysis take place?
A) Mitochondrial matrix
B) Cytoplasm
C) Chloroplast
D) Nucleus
3. What is the initial substrate in glycolysis?
A) Pyruvate
B) Glucose
C) Acetyl-CoA
D) ATP
4. How many ATP molecules are used in the energy investment phase of glycolysis?
A) 1
B) 2
C) 4
D) 6
5. What is the net gain of ATP molecules per glucose molecule during glycolysis?
A) 1
B) 2
C) 4
D) 6
6. What are the end products of glycolysis?
A) Glucose and oxygen
B) Pyruvate, ATP, and NADH
C) Acetyl-CoA and CO₂
D) Lactate and ethanol
7. Which molecule is reduced during glycolysis?
A) ATP
B) NAD+
C) Pyruvate
D) Glucose
8. How many NADH molecules are produced per glucose molecule in glycolysis?
A) 1
B) 2
C) 4
D) 6
9. What is the role of ATP in the early steps of glycolysis?
A) ATP is produced
B) ATP is hydrolyzed to provide energy
C) ATP acts as a final electron acceptor
D) ATP transports electrons
10. Which enzyme catalyzes the conversion of glucose to glucose-6-phosphate?
A) Hexokinase
B) Phosphofructokinase
C) Pyruvate kinase
D) Lactate dehydrogenase
11. Which step in glycolysis is considered the rate-limiting step?
A) Conversion of glucose to glucose-6-phosphate
B) Conversion of fructose-6-phosphate to fructose-1,6-bisphosphate
C) Conversion of phosphoenolpyruvate to pyruvate
D) Conversion of pyruvate to acetyl-CoA
12. During glycolysis, glucose is split into two molecules of:
A) Glyceraldehyde-3-phosphate
B) Pyruvate
C) Fructose-6-phosphate
D) Glucose-6-phosphate
13. How many carbon atoms are in one molecule of pyruvate produced at the end of glycolysis?
A) 2
B) 3
C) 4
D) 6
14. What happens to pyruvate if oxygen is not present?
A) It enters the Krebs cycle
B) It undergoes fermentation
C) It is converted into acetyl-CoA
D) It is converted into glucose
15. Which coenzyme accepts electrons during glycolysis?
A) NAD+
B) FAD
C) ATP
D) Coenzyme A
16. What is substrate-level phosphorylation in glycolysis?
A) The use of light energy to produce ATP
B) Direct synthesis of ATP by transferring a phosphate group
C) Production of ATP by the electron transport chain
D) Breaking down of glucose into pyruvate
17. Which molecule activates phosphofructokinase, the key enzyme in glycolysis?
A) ATP
B) ADP
C) Citrate
D) NADH
18. How many steps are there in the glycolysis pathway?
A) 5
B) 7
C) 10
D) 12
19. What is the function of glyceraldehyde-3-phosphate dehydrogenase in glycolysis?
A) It converts glucose to glucose-6-phosphate
B) It produces NADH by oxidizing glyceraldehyde-3-phosphate
C) It converts pyruvate into acetyl-CoA
D) It synthesizes ATP from ADP
20. Which of the following best describes glycolysis?
A) An aerobic process requiring oxygen
B) An anaerobic process that does not require oxygen
C) A process that occurs in the mitochondria
D) A process that produces oxygen as a byproduct
Answer Key
1. A – The breakdown of glucose into pyruvate
2. B – Cytoplasm
3. B – Glucose
4. B – 2
5. B – 2
6. B – Pyruvate, ATP, and NADH
7. B – NAD+
8. B – 2
9. B – ATP is hydrolyzed to provide energy
10. A – Hexokinase
11. B – Conversion of fructose-6-phosphate to fructose-1,6-bisphosphate
12. A – Glyceraldehyde-3-phosphate
13. B – 3
14. B – It undergoes fermentation
15. A – NAD+
16. B – Direct synthesis of ATP by transferring a phosphate group
17. B – ADP
18. C – 10
19. B – It produces NADH by oxidizing glyceraldehyde-3-phosphate
20. B – An anaerobic process that does not require oxygen
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